Coding

Part:BBa_K1745002:Design

Designed by: Benjamin Hsu   Group: iGEM15_UChicago   (2015-09-17)


AANAT Expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 509
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One thing that was uncertain was whether the AANAT could be secreted after expression, and if it could be secreted, whether it could retain its activity or not. We had thought about adding secretion pathway signals such as the Tat and Sec signal to the construct, and we had also thought about using addition methods such as adding phage shock protein genes and Kil genes, but in the end we decided against it because it would be hard to assay. We resolved to put these other genes on separate constructs.


Source

Homo Sapiens (Human)

References